From the November/December 2012 Issue
uAnalyze: Web-Based High-Resolution DNA Melting Analysis with Comparison to Thermodynamic Predictions
By Zachary L. Dwight, Robert Palais, Carl T. Wittwer
uAnalyzeSM is a web-based tool for analyzing high-resolution melting data of PCR products. PCR product sequence is input by the user and recursive nearest neighbor thermodynamic calculations used to predict a melting curve similar to uMELT (http://www.dna.utah.edu/umelt/umelt.html). Unprocessed melting data are input directly from LightScanner-96, LS32, or HR-1 data files or via a generic format for other instruments. A fluorescence discriminator identifies low intensity samples to prevent analysis of data that cannot be adequately normalized. Temperature regions that define fluorescence background are initialized by prediction and optionally adjusted by the user. Background is removed either as an exponential or by linear baseline extrapolation.
Editorials and Announcements
- We are pleased to announce that Ying Xu of the Department of Biochemistry and Molecular Biology and the Institute of Bioinformatics at the University of Georgia is the new Editor in Chief of the IEEE/ACM Transactions on Computational Biology and Bioinformatics starting in 2013.
- eBooks of issues of TCBB can now be downloaded from the Computer Society Digital Library
- EIC Editorial by M.-F. Sagot (March/April 2011)
- EIC Editorial by M.-F. Sagot (January/February 2011)
- Bioinformatics and Computational Systems Biology (July/August 2012)
- High Performance Computational Systems Biology (May/June 2012)
- Special Section on Bioinformatics Research and Applications (July/August 2011)
- Special Focus on Bioinformatics and Systems Biology (March/April 2011)
- Special Section on Bioinformatics Research and Applications (Oct-Dec 2010)
Access All Recently Published TCBB Articles
Subscribe to the RSS feed of latest TCBB content added to the digital library
Sign up for the Transactions Connection newsletter.
TCBB is a joint publication of the IEEE Computer Society, Association for Computing Machinery, IEEE Computational Intelligence Society, and the IEEE Engineering in Medicine and Biology Society.
Published in cooperation with: IEEE Control Systems Society
Indexed in MEDLINE®/PubMed® & ISI
IEEE/ACM Transactions on Computational Biology and Bioinformatics (TCBB) is a bimonthly journal that publishes archival research results related to the algorithmic, mathematical, statistical, and computational methods that are central in bioinformatics and computational biology.
Read the full scope of TCBB