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2015 IEEE International Conference on Bioinformatics and Biomedicine (BIBM) (2015)
Washington, DC, USA
Nov. 9, 2015 to Nov. 12, 2015
ISBN: 978-1-4673-6798-1
pp: 1102-1108
Julia Warnke-Sommer , Department of Computer Science, University of Nebraska Omaha, Department of Pathology and Microbiology, University of Nebraska Medical Center, USA
Ishwor Thapa , Department of Computer Science, University of Nebraska Omaha, Department of Pathology and Microbiology, University of Nebraska Medical Center, USA
Hesham Ali , Department of Computer Science, University of Nebraska Omaha, Department of Pathology and Microbiology, University of Nebraska Medical Center, USA
ABSTRACT
Next generation sequencing (NGS) has become the platform of numerous biomedical applications. The study of viral genomes using NGS technologies has led to the characterization of viral species in numerous environments including the human gut microbiome and plant hosts. Many viral genomes are circular or have terminally redundant ends. Circular or linear viral genomes with indeterminate starting and ending points pose a challenge for NGS assemblers, which may erroneously duplicate sections of these genomes. The length of an assembly, often characterized by the N50 length, is frequently used as an indication of an assembly's completeness and even quality. In this paper, we show that the longest contig produced by various assemblers is not always the best assembly for circular or terminally redundant phage genomes and may represent erroneously repeated genomic regions. Results demonstrate that assembly tools may even produce assembled genomes of different lengths for the same species, depending on content inaccurately repeated, leading to results that might be confusing to or inaccurately used by a researcher. To overcome this problem, we introduce strategies for using coverage depth to identify inaccurately repeated content in circular or terminally redundant phage genomes. We conclude the paper by providing the results of assembling two bacteriophage genomes and a bacteriophage metagenomics dataset, highlighting the impact of using the proposed strategies.
INDEX TERMS
Viral genome assembly, Next generation sequencing, Assembly validation
CITATION

J. Warnke-Sommer, I. Thapa and H. Ali, "Next generation sequence assembler mis-assembly of phage genomes with terminal redundancy," 2015 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), Washington, DC, USA, 2015, pp. 1102-1108.
doi:10.1109/BIBM.2015.7359836
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