This paper present a new method and model to analysis the venture investment decision based on the signaling game. We analyze and describe the venture investment decision making of two-period using mathematics models under the uncertainty to establish our option value model of venture investment. We analyze the asymmetric information between venture enterprises and venture investment corporations. Boil down the game between venture enterprises and venture investment corporations to signaling game under the incompleteness information. Get a solution of combination perfect Bayesian equilibrium.