Following the agile philosophy that all core features of a system need an automated test harness, performance requirements also need such a check when they are essential for the success of a project. The purpose of this paper is to describe a tool, J3DPerfUnit, which supports automated performance testing for Java3D applications in agile environments. We elicited tool requirements from domain experts through a survey and evaluated J3DPerfUnit using code from our partner?s bioinformatics project and Java3D official tutorials. The evaluation pointed out that the tool is effective in detecting performance problems and in identifying where they come from when loading/unloading a 3D object.