Bridge fatigue load vehicles model is the precondition of the fatigue checking computations, and must be lucubrated. According to the traffic investigation in Shanghai and Guangzhou city, analyzed representative vehicles axle weight and frequency. Based on the linearity Miner fatigue cumulative damage rule, progressed equivalent vehicles axle weight and wheelbase, predigested the representative vehicles to two-axle vehicle or three-axle vehicle fatigue model, where the two-axle vehicle’s former axle weight and back axle weight are 35kN and 80kN accordingly, wheelbase is 4.3m, and it is possessed 14.41% of total traffic, the three-axle vehicle’s former axle weight, middle axle weight and back axle weight are 34kN, 88kN and 108kN accordingly, former wheelbase and back wheelbase are 3.7m and 5.5m accordingly, and it is possessed 3.75% of total traffic. The checking computations results indicated that the model operated conveniently and had quite high precision. Advanced the apply condition of the Fatigue Load Vehicles Mode.