A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries

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2007 Frontiers in the Convergence of Bioscience and Information Technologies

Jeju Island, Korea

October 11-October 13

ISBN: 978-0-7695-2999-8

	ASCII Text	x
	Duwoon Kim, Seok-Ryel Kim, Donal F. Day, Myung-Joo Oh, "A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries," Frontiers in the Convergence of Bioscience and Information Technologies, pp. 64-68, 2007 Frontiers in the Convergence of Bioscience and Information Technologies, 2007.

	BibTex	x
	@article{ 10.1109/FBIT.2007.73, author = {Duwoon Kim and Seok-Ryel Kim and Donal F. Day and Myung-Joo Oh}, title = {A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries}, journal ={Frontiers in the Convergence of Bioscience and Information Technologies}, volume = {0}, year = {2007}, isbn = {978-0-7695-2999-8}, pages = {64-68}, doi = {http://doi.ieeecomputersociety.org/10.1109/FBIT.2007.73}, publisher = {IEEE Computer Society}, address = {Los Alamitos, CA, USA}, }

	RefWorks Procite/RefMan/Endnote	x
	TY - CONF JO - Frontiers in the Convergence of Bioscience and Information Technologies TI - A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries SN - 978-0-7695-2999-8 SP64 EP68 A1 - Duwoon Kim, A1 - Seok-Ryel Kim, A1 - Donal F. Day, A1 - Myung-Joo Oh, PY - 2007 VL - 0 JA - Frontiers in the Convergence of Bioscience and Information Technologies ER -

DOI Bookmark: http://doi.ieeecomputersociety.org/10.1109/FBIT.2007.73

SephadexTM bead agarose electrophoresis (SBAE) was used to enrich the higher dextran binding phages. Phage collection obtained after the 2nd round of SBAE was 3.5 fold higher color intensity than that of phage collections obtained after the 1st round. DNA sequencing of phage collection (SBAE-2R) confirmed that human origin antibody was present. The PCR products of , light chains and heavy chain from phage collection (SBAE-2R) were approximately 420 bp, 550 bp and 600 bp.

Citation:

Duwoon Kim, Seok-Ryel Kim, Donal F. Day, Myung-Joo Oh, "A Novel Screening Method of Dextran Binding Antibody Using Phage Display Libraries," fbit, pp.64-68, 2007 Frontiers in the Convergence of Bioscience and Information Technologies, 2007

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