The growth of bioinformatics has resulted in datasets with new characteristics. The DNA sequences typically contain a large number of items. From them biologists assemble a whole genome of species based on frequent concatenate sequences, which ordinarily have hundreds of items.

Such datasets pose a great challenge for existing frequent pattern discovery algorithms. Almost all of them are Apriori-like and so have an exponential dependence on the average sequence length. PrefixSpan is the most efficient algorithm, which presented the projection-based sequential patterngrowth approach. However it grows sequential patterns by exploring length-1 frequent patterns and so is not suitable for biological dataset with long frequent concatenate sequences.

In this paper, we propose two novel algorithms, called MacosFSpan and MacosVSpan, to mine maximal frequent concatenate sequences. They are specially designed to handle datasets having long frequent concatenate sequences. Our performance study shows that MacosFSpan outperforms the traditional methods with length-1 sequences exploration and MacosVSpan is more efficient than MacosVSpan.