Depending on the duration of exposure at supraphysiological temperatures, cellular proteins and organelles would suffer structural alternation and irreversible denaturation which may induce cell death. In the present study, a commercial hotplate was used to provide a constant temperature to heat the human embryonic kidney cells (293T).??Heat transfer model were performed to evaluate thermal equilibrium time required for the cell samples.??The survival of the human embryonic kidney cell line was investigated herein and the frequency factor and activation energy of 293T cells in the Arrhenius model for the thermal induced cell injury were obtained as A=5.95?1030s-1 and ΔE=211.79kJ/mol, respectively.??It can also be observed patently that the cell membrane was ruptured when the cell was exposing such a supraphysiological temperature.