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2008 International Conference on BioMedical Engineering and Informatics
Human GITRLaa50-177 Expressed in Bac-to-Bac Baculovirus Expression System
May 27-May 30
ISBN: 978-0-7695-3118-2
Glucocorticoid induced tumor necrosis factor receptor ligand (GITRL) is a type II transmembrane protein with homology to TNF receptor superfamily members, and its human receptor (hGITR), an ortholog of the recently discovered murine glucocorticoid-induced TNFR-related (mGITR) protein. Ligation of murine GITR/GITRL can enhance immunity reactions via several signal pathways. However, so far the certain function of hGITR is not clear. In this study, we first used Bac-to-Bac baculovirus expression system to express hGITRLaa50-177 recombinant protein, then identified the recombinant protein by SDS-PAGE, western blot and indirect immunofluorescence staining.
Index Terms:
GITRL, recombinant protein
Citation:
Li Tang, Sheng-Jun Wang, Chao-Ming Mao, Jun Chen, Zheng-Jun Hu, Jun-Feng Bao, Qi-Xiang Shao, Hua-Xi Xu, "Human GITRLaa50-177 Expressed in Bac-to-Bac Baculovirus Expression System," bmei, vol. 1, pp.910-914, 2008 International Conference on BioMedical Engineering and Informatics, 2008
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