To study the antibiotic susceptibility of eleven vaginal probiotic lactobacilli, the disc diffusion method in LAPTg agars was performed. The results had shown that all strains were resistance to tobramycin, lomefloxacin, nalidixic scid, ofloxacin, enoxacin, ciprofloxacin, norfloxacin and fleroxacin. There of eight were resistance to sparfloxacin, six were resistance to gentamicin and netilmicin. All the lactobacilli were sensitive to β-lactamade inhibitors and macrolides whereas resistant to tinidazole. Therefore, the susceptibility to some antibiotics presented similar. On the other hand, the sensitivity to other antibiotics depended on strains. Our results indicated that resistant strains of lactobacilli against tinidazole could be combinated with tinidazole for the restoration of the normal urogenital flora and curing of urinary tract infections or bacterial vaginosis.