We propose a fast reconstruction algorithm for non-adherent living cell images from micro-rotational confocal microscopy. This procedure is called bi-protocol. Its key point consists in coupling micro-rotation data and conventional z-stack. Doing so, the estimation of the micro-rotation slice positions becomes a slice-to-volume registration allowing to reduce drastically the computing time. The validation of our procedure is performed on real data which gives significant improvement compared with the conventional z-stacking imaging.