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2008 International Conference on BioMedical Engineering and Informatics
The Analysis of Chemotherapy Resistance in Human Non-small Cell Lung Cancer Cell Line with an Integrated Microfluidic Device
May 27-May 30
ISBN: 978-0-7695-3118-2
ASCII Text
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Siyan Wang, Lichuan Zhang, Li Jiang, Bingcheng Lin, Qi Wang, "The Analysis of Chemotherapy Resistance in Human Non-small Cell Lung Cancer Cell Line with an Integrated Microfluidic Device," BioMedical Engineering and Informatics, International Conference on, vol. 2, pp. 691-697, 2008 International Conference on BioMedical Engineering and Informatics, 2008.
 
 
BibTex
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@article{ 10.1109/BMEI.2008.164,
author = {Siyan Wang and Lichuan Zhang and Li Jiang and Bingcheng Lin and Qi Wang},
title = {The Analysis of Chemotherapy Resistance in Human Non-small Cell Lung Cancer Cell Line with an Integrated Microfluidic Device},
journal ={BioMedical Engineering and Informatics, International Conference on},
volume = {2},
year = {2008},
isbn = {978-0-7695-3118-2},
pages = {691-697},
doi = {http://doi.ieeecomputersociety.org/10.1109/BMEI.2008.164},
publisher = {IEEE Computer Society},
address = {Los Alamitos, CA, USA},
}
 
 
RefWorks Procite/RefMan/Endnote
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TY - CONF
JO - BioMedical Engineering and Informatics, International Conference on
TI - The Analysis of Chemotherapy Resistance in Human Non-small Cell Lung Cancer Cell Line with an Integrated Microfluidic Device
SN - 978-0-7695-3118-2
SP691
EP697
A1 - Siyan Wang,
A1 - Lichuan Zhang,
A1 - Li Jiang,
A1 - Bingcheng Lin,
A1 - Qi Wang,
PY - 2008
KW - GRP78
KW - Microfluidic device
KW - Immunofluorescence
KW - VP-16
KW - Chemotherapy resistance
VL - 2
JA - BioMedical Engineering and Informatics, International Conference on
ER -
 
Microchip-based systems have many desirable characteristics and can be used in much cellular biological analysis. This work studied the correlation between the expression of Glucose-regulated protein-78 (GRP78) and anticancer drug VP-16 in human non-small cell lung cancer cell line SK-MES-1 using an integrated microfluidic device. A concentration gradient of stimulator (A23187) was generated in the upstream network of the device to induce the cells cultured in the downstream. Then the cells were treated by VP-16. The expression of GRP78 was detected by immunofluorescence. Apoptosis was assessed morphologically by 4’-6-Diamidino-2-phenylindol??nuclear staining for the cells treated by VP-16. The expressions of GRP78 for the A23187-induced cells increased. After the treatment by VP-16, the percentage of apoptotic cells decreased with the increasing of A23187 concentration. GRP78 plays an important role in the resistance to VP-16 in SK-MES-1. This system offers a unique platform to characterize cellular responses in a high-throughput fashion.
Index Terms:
GRP78, Microfluidic device, Immunofluorescence, VP-16, Chemotherapy resistance
Citation:
Siyan Wang, Lichuan Zhang, Li Jiang, Bingcheng Lin, Qi Wang, "The Analysis of Chemotherapy Resistance in Human Non-small Cell Lung Cancer Cell Line with an Integrated Microfluidic Device," bmei, vol. 2, pp.691-697, 2008 International Conference on BioMedical Engineering and Informatics, 2008
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