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Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04)
Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR
Taichung, Taiwan, ROC
May 19-May 21
ISBN: 0-7695-2173-8
ASCII Text
x 
 
K. Rocha, C. Medeiros, M. Monteiro, L. Gon?alves, P. Marinho, "Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR," Bioinformatic and Bioengineering, IEEE International Symposium on, pp. 149, Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04), 2004.
 
 
BibTex
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@article{ 10.1109/BIBE.2004.1317337,
author = {K. Rocha and C. Medeiros and M. Monteiro and L. Gon?alves and P. Marinho},
title = {Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR},
journal ={Bioinformatic and Bioengineering, IEEE International Symposium on},
volume = {0},
year = {2004},
isbn = {0-7695-2173-8},
pages = {149},
doi = {http://doi.ieeecomputersociety.org/10.1109/BIBE.2004.1317337},
publisher = {IEEE Computer Society},
address = {Los Alamitos, CA, USA},
}
 
 
RefWorks Procite/RefMan/Endnote
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TY - CONF
JO - Bioinformatic and Bioengineering, IEEE International Symposium on
TI - Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR
SN - 0-7695-2173-8
SP
EP
A1 - K. Rocha,
A1 - C. Medeiros,
A1 - M. Monteiro,
A1 - L. Gon?alves,
A1 - P. Marinho,
PY - 2004
KW - null
VL - 0
JA - Bioinformatic and Bioengineering, IEEE International Symposium on
ER -
 
K. Rocha, Universidade Federal do Rio Grande do Norte, Brazil
C. Medeiros, Universidade Federal do Rio Grande do Norte, Brazil
M. Monteiro, Universidade Federal do Rio Grande do Norte, Brazil
L. Gon?alves, Universidade Federal do Rio Grande do Norte, Brazil
P. Marinho, Universidade Federal do Rio Grande do Norte, Brazil
We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a virus diagnosis.
Citation:
K. Rocha, C. Medeiros, M. Monteiro, L. Gon?alves, P. Marinho, "Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR," bibe, pp.149, Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04), 2004
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