Various techniques in analyzing palmprint have been proposed but to the best of our knowledge, none has been studied on the selection and division of the region-of-interest (ROI). Previous methods were always applied only to a fixed size square region chosen as the central part of the palm, which were then divided into square blocks for extraction of local features. In this paper, we proposed a new method in locating and segmenting the ROI for palmprint analysis, where the selected region varies with the size of the palm. Instead of square blocks, the region is divided into sectors of elliptical half-rings, which are less affected by misalignment due to rotational error. More importantly, our arrangement of the feature vectors ensures that only features extracted from the same spatial region of two aligned palms will be compared with each other. Encouraging results obtained favor the use of this method in the future development of palmprint analysis techniques.