Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR

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	Similar Articles Articles by K. Rocha Articles by C. Medeiros Articles by M. Monteiro Articles by L. Gon?alves Articles by P. Marinho

Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04)

Taichung, Taiwan, ROC

May 19-May 21

ISBN: 0-7695-2173-8

	ASCII Text	x
	K. Rocha, C. Medeiros, M. Monteiro, L. Gon?alves, P. Marinho, "Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR," Bioinformatic and Bioengineering, IEEE International Symposium on, pp. 149, Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04), 2004.

	BibTex	x
	@article{ 10.1109/BIBE.2004.1317337, author = {K. Rocha and C. Medeiros and M. Monteiro and L. Gon?alves and P. Marinho}, title = {Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR}, journal ={Bioinformatic and Bioengineering, IEEE International Symposium on}, volume = {0}, year = {2004}, isbn = {0-7695-2173-8}, pages = {149}, doi = {http://doi.ieeecomputersociety.org/10.1109/BIBE.2004.1317337}, publisher = {IEEE Computer Society}, address = {Los Alamitos, CA, USA}, }

	RefWorks Procite/RefMan/Endnote	x
	TY - CONF JO - Bioinformatic and Bioengineering, IEEE International Symposium on TI - Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR SN - 0-7695-2173-8 SP EP A1 - K. Rocha, A1 - C. Medeiros, A1 - M. Monteiro, A1 - L. Gon?alves, A1 - P. Marinho, PY - 2004 KW - null VL - 0 JA - Bioinformatic and Bioengineering, IEEE International Symposium on ER -

K. Rocha, Universidade Federal do Rio Grande do Norte, Brazil

C. Medeiros, Universidade Federal do Rio Grande do Norte, Brazil

M. Monteiro, Universidade Federal do Rio Grande do Norte, Brazil

L. Gon?alves, Universidade Federal do Rio Grande do Norte, Brazil

P. Marinho, Universidade Federal do Rio Grande do Norte, Brazil

DOI Bookmark: http://doi.ieeecomputersociety.org/10.1109/BIBE.2004.1317337

We propose a specialist software to diagnose viral disease in plants. Our strategy is to align nucleotide sequences of plant virus to discover specie-specific regions of genes of the viral genomes, so as to design a primer. The program designs oligo-nucleotide primers used for polymerase chain reaction (PCR), a very cheap diagnosis technique. The user can specify (or use default) constraints for primer and amplified product lengths, as percentage of G+C, absolute or relative melting temperatures, and primer 3' nucleotides. The program screens candidate primer sequences with displayed user-specifiable parameters in order to help minimizing nonspecific priming and primer secondary structure. We tested this tool by designing two specific primers which were used to amplify known viral species, then used to perform a virus diagnosis.

Citation:

K. Rocha, C. Medeiros, M. Monteiro, L. Gon?alves, P. Marinho, "Design of Specie-Specific Primers for Virus Diagnosis in Plants with PCR," bibe, pp.149, Fourth IEEE Symposium on Bioinformatics and Bioengineering (BIBE'04), 2004

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