As a requisite to the complete e-business revolution, Negotiation Support System (NSS) today hasn't been widely adopted in real e-Business negotiation practices, despite years of research on it. On the basis of our former work on NSS, this paper focuses on the implementation of theories, models and technologies into practice. The web-based negotiation support framework presented in this paper is practical, in that: a) different negotiation protocols can be adopted; b) business negotiations can be supported dynamically according to participants' settings; c) the framework offers easy-to-maintain business logic and decision models; d) the system may be hopefully integrated into corporate information systems. A common negotiation protocol is designed; and a linear negotiation mediation models is given. An implementation that enables the outstanding features of this framework is discussed as well.