CSDL Home IEEE/ACM Transactions on Computational Biology and Bioinformatics 2011 vol.8 Issue No.05 - September/October
Summarizing Probe Intensities of Affymetrix GeneChip 3' Expression Arrays Taking into Account Day-to-Day Variability
Issue No.05 - September/October (2011 vol.8)
Angela Simeone , (BIOTEC), Technische Universität Dresden
Sandra Healy , National University of Ireland, Galway
Antonella Isacchi , Nerviano Medical Sciences, Nerviano
Roberta Bosotti , Nerviano Medical Sciences, Nerviano
DOI Bookmark: http://doi.ieeecomputersociety.org/10.1109/TCBB.2010.82
Microarray experiments are affected by several sources of variability. The paper demonstrates the major role of the day-to-day variability, it underlines the importance of a randomized block design when processing replicates over several days to avoid systematic biases and it proposes a simple algorithm that minimizes the day dependence.
Gene expression, statistical analysis, Affymetrix microarray, experimental variability.
Angela Simeone, Sandra Healy, Antonella Isacchi, Roberta Bosotti, "Summarizing Probe Intensities of Affymetrix GeneChip 3' Expression Arrays Taking into Account Day-to-Day Variability", IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol.8, no. 5, pp. 1425-1430, September/October 2011, doi:10.1109/TCBB.2010.82