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Issue No.02 - April-June (2009 vol.6)
pp: 1
G.H. Weber , Lawrence Berkeley Nat. Lab., Berkeley, CA, USA
O. Rubel , Dept. of Comput. Sci., Univ. of Kaiserslautern, Kaiserslautern, Germany
A.H. DePace , Med. Sch., Dept. of Syst. Biol., Harvard Univ., Boston, MA, USA
C.C. Fowlkes , Dept. of Comput. Sci., Univ. of California, Irvine, CA, USA
S.V.E. Keranen , Lawrence Berkeley Nat. Lab., Berkeley, CA, USA
C.L. Luengo Hendriks , Centre for Image Anal., Uppsala Univ., Uppsala, Sweden
H. Hagen , Dept. of Comput. Sci., Univ. of Kaiserslautern, Kaiserslautern, Germany
D.W. Knowles , Lawrence Berkeley Nat. Lab., Berkeley, CA, USA
J. Malik , Comput. Sci. Div., Univ. of California, Berkeley, CA, USA
M.D. Biggin , Lawrence Berkeley Nat. Lab., Berkeley, CA, USA
B. Hamann , Dept. of Comput. Sci., Univ. of California, Davis, CA, USA
ABSTRACT
During animal development, complex patterns of gene expression provide positional information within the embryo. To better understand the underlying gene regulatory networks, the Berkeley Drosophila Transcription Network Project (BDTNP) has developed methods that support quantitative computational analysis of three-dimensional (3D) gene expression in early Drosophila embryos at cellular resolution. We introduce PointCloudXplore (PCX), an interactive visualization tool that supports visual exploration of relationships between different genes' expression using a combination of established visualization techniques. Two aspects of gene expression are of particular interest: 1) gene expression patterns defined by the spatial locations of cells expressing a gene and 2) relationships between the expression levels of multiple genes. PCX provides users with two corresponding classes of data views: 1) physical views based on the spatial relationships of cells in the embryo and 2) abstract views that discard spatial information and plot expression levels of multiple genes with respect to each other. Cell selectors highlight data associated with subsets of embryo cells within a View. Using linking, these selected cells can be viewed in multiple representations. We describe PCX as a 3D gene expression visualization tool and provide examples of how it has been used by BDTNP biologists to generate new hypotheses.
INDEX TERMS
genetics, bioinformatics, cellular biophysics, embryo cells, three-dimensional gene expression, visual exploration, gene regulatory networks, Drosophila embryos, PointCloudXplore, visualization tool, Gene expression, Embryo, Data visualization, Computer science, Animals, Computer networks, Spatial resolution, Bioinformatics, Biology computing, Cellular networks, scatter plots., Interactive data exploration, three-dimensional gene expression, spatial expression patterns, information visualization, visualization, physical views, multiple linked views, brushing, Picture/Image Generation, General
CITATION
G.H. Weber, O. Rubel, A.H. DePace, C.C. Fowlkes, S.V.E. Keranen, C.L. Luengo Hendriks, H. Hagen, D.W. Knowles, J. Malik, M.D. Biggin, B. Hamann, "Visual Exploration of Three-Dimensional Gene Expression Using Physical Views and Linked Abstract Views", IEEE/ACM Transactions on Computational Biology and Bioinformatics, vol.6, no. 2, pp. 1, April-June 2009, doi:10.1109/TCBB.2007.70249
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