Although E-Learning has grown into a revolutionary way of learning and is currently widely accepted, it still faces many problems, especially in its implementations. The research community has believed that an E-Learning ecosystem is the next generation E-Learning. Nowadays, the current trend of Web 2.0 has a strong impact on E-Learning. In this paper, mashups are used to build an E-Learning ecosystem. A mashup approach to an E-Learning ecosystem enhances the flourish and sustainability of E-Learning in a productive and cost-effective way. This paper describes the concepts of vertical stack, users and operations in a mashup E-Learning ecosystem. A prototype system, named Jampots, has been built. Jampots has features such as openness, reusability, lightweight structure, end user-orientation and self-regulation.